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 Small RNA sequencing



Service introduction




        Small RNAs (sRNA) are important regulators of life activities, playing important roles in gene expression regulation, individual development, metabolism, and the occurrence of diseases. The Illumina sequencing platform can perform deep sequencing of all sRNA in the sample to achieve qualitative and quantitative research purposes. Each sample can obtain more than 10 million sRNA sequencing sequences. Through massive parallel sequencing, it can discover, identify, and quantify the small RNA map at the whole genome level of any species, the excavation of new mRNA molecules, the prediction of target genes, identification, and differential expression analysis between samples.

 

Technical procedures




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Sample requirements




 

实验类型

RNA样品总量

样品浓度和纯度

 样品质量

样品保存

样品运输

Small RNA测序

一次性提供至少3 μg高质量的总 RNA

样品浓度 >100 ng/μl,OD260/280 介于 1.8-2.0 之间,无肉眼可见污染 

使用 Agilent Bioanalyzer 仪器检测,则获得的总的 RNA 28S:18S >1,RIN>7.5

请选择乙醇、DEPC 水保存样品,并在样品信息单中注明 

样品请置于 1.5 ml 管中,并使用封口膜封好,放置足够的干冰运输

 

Experiment cycle and sequencing strategy



 

实验类型

测序策略

 数据量

实验周期

Small RNA测序

HiseqSE50 

10M

样品质检合格后,30-50 个工作日

 


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